Table 1.
Tryptophan concentrations (μg mL−1) in unstimulated and TLR agonist stimulated whole blood supernatants from healthy controls and IBS patients.
| Control |
IBS |
|||
|---|---|---|---|---|
| TLR stimulation | Tryptophan | p | Tryptophan | p |
| Unstimulated | 13.5 ± 0.2 | N/A | 13.7 ± 0.3 | N/A |
| 1/2 (Pam3Cys) | 11.7 ± 0.2 | *** | 12.0 ± 0.3 | $$ |
| 2 (HKLM) | 13.1 ± 0.3 | – | 12.1 ± 0.3 | $$ |
| 3 (Poly I:C) | 11.8 ± 0.3 | *** | 12.3 ± 0.2 | $$ |
| 4 (LPS) | 13.7 ± 0.4 | – | 13.7 ± 0.4 | – |
| 5 (Flagellin) | 13.3 ± 0.3 | – | 13.3 ± 0.3 | – |
| 6/2 (FSL1) | 12.6 ± 0.2 | * | 13.2 ± 0.3 | – |
| 7 (Imiquimod) | 13.0 ± 0.2 | – | 13.0 ± 0.2 | – |
| 8 (ssRNA40) | 12.6 ± 0.3 | * | 12.9 ± 0.2 | – |
| 9 (ODN2006) | 13.4 ± 0.5 | – | 13.0 ± 0.5 | – |
Data are expressed as means ± SEM. Statistical differences between stimulated and unstimulated groups were determined using Bonferroni post hoc test. *p < 0.05, Healthy control unstimulated vs. TLR agonist stimulated; ***p < 0.001, healthy control unstimulated vs. TLR agonist Stimulated; $$ p < 0.01, IBS unstimulated vs. stimulated; – p > 0.05.