Table 1.

Concentration of nicotine, cotinine, and trans-3′-hydroxycotinine in the urine of nicotine consuming mice^a

Group	Treatmentb	Time	Nicotine (ng/ml)		Cotinine (ng/ml)		trans 3′-Hydroxycotinine (ng/ml)
			Mean	Range	Mean	Range	Mean	Range
2	Nicotine 0–46 weeks/saline	1–2 weeksc (n= 26)	1360 ± 1040	65 – 3570	1260 ± 1240	116 – 4700	12200 ± 9010	1600 – 34000
		5 month (n=14)	1270 ± 1170	217 – 3620	4400 ± 3200	450 – 10600	27900 ± 9820	17100 – 49600
4	Nicotine 0–46 weeks/NNK	1–2 weeks (n = 24)	1900 ± 2550	53 – 7700	4790 ± 5860	70 – 17500	18900 ± 13500	4300 – 48000
		5 months (n=15)	2170 ± 2760	156 – 10,500	5700 ± 4300	930 – 16000	42900 ± 19400	16600 – 74400
5	Nicotine 0–2 weeks/NNKa	1–2 weeks (n=25)	980 ± 930	22 – 2870	3520 ± 6220	150 – 3120	17000 ± 10500	2920 – 37500
		5 months	ndd		nd		nd
6	Nicotine 2–46 weeks/NNKb	1–2 weeks	nd		nd		nd
		5 months (n= 20)	840 ± 714	62 – 2560	5910 ± 4140	820 – 14700	37800 ± 19400	9750 – 83400

^aUrine obtained from individual mice was analyzed by LC/MS/MS, values are provided for two time points for individual mice from which urine was collected.

^bThe protocol is as illustrated in Fig. 1, beginning at 5 weeks of age female A/J mice (Groups 2,4,5) were provided 0.44 μmol/ml nicotine in the drinking water, after 2 weeks animals in Groups 4, 5 and 6 were administered a single dose of NNK (10 μmol), nicotine was discontinued for Group 5 and initiated in Group 6 at the time of the NNK injection

^cUrine was collected from a single mouse between 7 and 14 days of nicotine consumption the remaining samples were from a single collection per mouse from 2 to 7 days.

^dnd, not determined, animals were not consuming nicotine at this time