Fig. 3.

miRNA profile in the heart of ZL and ZO rats. The miRNA was isolated with an mirVana miRNA Isolation Kit (Ambion Inc.) from fresh-frozen tissues (n = 3 for each group), and was labeled with a FlashTag™ Biotin HSR RNA Labeling Kit. An Affymetrix miRNA GeneChip was used for this study (46,228 probes comprising 7,815 probe sets, including controls). The probes on this chip are derived from the Sanger miRBase miRNA database v11 (April 15, 2008, http://microrna.sanger.ac.uk) [173]. Data analysis was performed by miRNA QC tool and Significance Analysis of Microarrays (SAM) software [174]. LV = Left ventricle. * p < 0.05 for ZL versus ZO.