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. Author manuscript; available in PMC: 2013 Apr 1.
Published in final edited form as: Dev Growth Differ. 2012 Apr;54(3):306–316. doi: 10.1111/j.1440-169X.2012.01342.x

Figure 2. Centrosomes leave the apical surface to initiate mitosis.

Figure 2

The intact nucleus is marked with NLS-tdTomato and an asterisk; centrosomes are marked with GFP-centrin and green arrows. The average distance from site of nuclear envelope breakdown to final position during mitosis is 16.5 μm (N = 15). All mitotic cells are observed to undergo apical rounding during mitosis (N = 62). NLS-tdTomato and GFP-centrin were introduced to neural tube cells by in-ovo electroporation at Hamburger/Hamilton stage 12 to 16. Neural tubes were sectioned and imaged less than 24 hours later on an Olympus confocal microscope. Images were taken every 7 minutes. Scale bar = 10 μm

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