FIG. 5.

HIF pathway activation in D-HF patients and miR-199a, miR-199b, and miR-210 regulation by hypoxia (HYP) and high glucose (HG) in vitro. A: mRNA expression level of the HIF family and HIF target genes were assayed by qRT-PCR in D-HF (n = 6) and ND-HF (n = 16) samples. *P ≤ 0.05, **P ≤ 0.01. B: Neonatal mouse cardiomyocytes (nCMs) and HCAECs were cultured in HYP (1% O₂), in HG (25 mmol/L), in HG followed by HYP (HG+HYP), or in normoxic and iso-osmotic control conditions. Next, total RNA was extracted and miR-199a, miR-199b, and miR-210 levels were assayed. Data are expressed using a log₂ scale (-ΔΔCt) by a heat map, where green and red colors indicate down- or upregulation, respectively. As a reference, miR-199a, miR-199b, and miR-210 levels observed in D-HF and ND-HF heart biopsies are indicated as well.