FIG. 3.
Effects of TCDC and GW4064 on [Ca2+]c. A: TCDC increased [Ca2+]c in the presence of 15 mmol/L glucose. To quantify the data, the AUC was calculated for a time interval of 300 s before and during addition of TCDC. Preincubation with guggulsterone blunted the stimulatory effect of TCDC (right). B: GW4064 had similar stimulatory effects on [Ca2+]c that were suppressed by guggulsterone (guggul., right). C: UDC did not alter the AUC in the presence of 15 mmol/L glucose. Representative experiments at left. The diagrams at right summarize the data and illustrate the AUC in the presence of 15 mmol/L glucose (G15, white bars), with TCDC or TCDC + guggulsterone (black bars in A), GW4064, or GW4064 + guggulsterone (hatched bars in B) or UDC (striped bar in C). n is given within each bar. **P ≤ 0.01, ***P ≤ 0.001.