Sequence specific target detection in extracellular experiments. (A) A scheme of sequence specific flare release in response to two different targets (green shown on the left and red shown on the right). (B, C) Fluorescence spectra of nano-flares in the presence of no target, actin target or survivin target. Actin and survivin flares are labeled with Cy5 and Cy3, respectively. (B) Normalized fluorescence spectra corresponding to Cy3 (survivin flare) signal, with excitation at 530 nm. (C) Normalized fluorescence spectra corresponding to Cy5 (actin flare) signal, with excitation at 630 nm.