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. Author manuscript; available in PMC: 2013 Feb 21.
Published in final edited form as: Anal Chem. 2012 Jan 30;84(4):2062–2066. doi: 10.1021/ac202648w

Figure 4.

Figure 4

Quantitative and qualitative determination of mRNA expression in living cells. (A) The detection of intracellular survivin mRNA by traditional nano-flares was compared to detection by multiplexed nano-flares, expressed as the ratio of survivin to actin. The cell associated fluorescence intensities were measured in HeLa cells using flow cytometry and expressed as a histogram. (B) In a similar experiment, the multiplexed nano-flares were used to compare the ratio of survivin to actin expression in HeLa and Jurkat cells. (C) The expression of survivin mRNA was measured as a function of survivin siRNA delivered by both the multiplexed nano-flare and RT-PCR. Error bars represent standard error of the mean from at least 3 independent experiments.