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. Author manuscript; available in PMC: 2013 Apr 27.
Published in final edited form as: Circ Res. 2012 Mar 22;110(9):1169–1173. doi: 10.1161/CIRCRESAHA.112.267716

Figure 2. CPCs asymmetrically segregate BrdU labeled chromatids.

Figure 2

A and B, BrdU intensity distribution among daughter nuclei in binucleated cells at first (A) and second (B) mitosis during chase in label retention assay. 70:30 distribution threshold for BrdU intensity ratio is shown as dotted box in panel B and events above this threshold are considered asymmetrically segregating cells. C and D, Representative binucleated cells with symmetric (C) and asymmetric (D) BrdU immuno-stain.