Figure 2. Fluorescence of egfp-containing cells of L. monocytogenes EGD-e wild type, ΔsigB and ΔrsbV mutants after transformation with pKSV7-P_lmo2230::egfp. Phase-contrast and fluorescence microscopy of corresponding fields were performed after 1 ml of stationary phase culture was centrifuged, resuspended in 50 μl of sterile PBS and 5 μl was smeared on the slides for visualization. Images are representative of a minimum of ten randomly selected fields captured for three biological replicates for each strain.