Figure 3. EGFP is expressed of L. monocytogenes EGD-e wild type but not in ΔsigB and ΔrsbV backgrounds. Western blot analyses were performed using protein extracts from stationary phase cultures (normalized to 5 mg ml⁻¹ total protein concentrations and 10 µl of these samples separated by SDS-PAGE) with chicken polyclonal anti-GFP antibody (Abcam) HRP-conjugated goat anti-chicken antibody (Promega) and chemiluminescent detection.