Figure 2. Root flavonoids act as a signal to induce the production of MiSSP7. Indirect immunolocalizations of MiSSP7 performed as described by Plett and colleagues (2011) on L. bicolor grown on cellophane in indirect contact with poplar roots (A) or dosed with water (B; control treatment) or with 100 µM of glucose (C), rhamnose (D), rutin (E) or quercitin (F) every 2 d for 1 week. MiSSP7 is only produced when in indirect contact with root tissue or when treated with the flavonoids rutin and quercitin. All experiments were performed in triplicate to ensure reproducibility. Scale bar = 70 µm.