Figure 3. Effects of conditioned medium on the incidence of SE. The number of somatic embryos per mm of root (mean ± SEM) was scored after lugol staining for the embryogenic hybrid (A) or for the recalcitrant C. intybus cv Pe´vèle (B) after 12 d (white bars) or 20 d (gray bars) of culture. The media were conditioned by culturing roots of the hybrid for 0, 2 and 4 d in standard conditions. Scoring of embryos was done on two independent experiments each involving ten root explants.