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. 2012 May 21;7(5):e37503. doi: 10.1371/journal.pone.0037503

Figure 5. Histopathological examination of treatment efficacy in traumatic ONFH by H&E staining, and immunohistochemical staining and semi-quantitative analysis of CD105.

Figure 5

(A) Representative image of a section of femoral head stained with H&E. Scale bar = 200 µm. In the treated femoral head, the number of empty lacunae decreased and hematopoietic tissue partially recovered, accompanied by an increase in the number of OBs, which was most significant in animals that received HGF-transgenic MSCs. a. empty lacuna; b. OBs. (B) Bar graph (left panel) represents the ratio of bone marrow cells to the area of bone marrow. A second bar graph (right panel) represents the ratio of empty lacunae to the area of trabeculae. (C) Immunohistochemical assays of CD105 expression. Scale bar = 20 µm. (D) Bar graphs represent the expression density of CD105 as unit area of bone marrow or unit area of bone trabeculae. After MSC transplantation, the expression of CD105 increased compared with that of untreated ONFH group, indicating the occurrence of revascularization. Quantification was based on at least 10 fields per section. *P<0.05.