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. 2012 May 21;7(5):e37297. doi: 10.1371/journal.pone.0037297

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Ma et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) U87 cells were double-stained for βig-h3 (red) and integrin α5 or β1 (green). (B) Expression of βig-h3 and integrin α5 and β1 subunits in U87 cell lysates. (C) Precipitates from βig-h3 immunocomplexes were detected for precipitated integrin α5 and β1 subunits. Mouse IgG was used as a negative control. Precipitates from α5 (D) or β1 (E) immunocomplexes were detected for precipitated βig-h3. Mouse IgG was used as a negative control. Bar = 50 um.