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. 2012 May 21;7(5):e37457. doi: 10.1371/journal.pone.0037457

Figure 4. The influences of AcMNPV infection on the starvation-induced autophagy in SL-PHP cells.

Figure 4

(A) Staining of MDC (A1–3) and Lyso-tracker Red (A4–6). (A1 and A4) Control SL-HP cells cultured in complete medium with 10% FBS, (A2 and A5) SL-HP cells starved for 6.5 h, (A3 and A6) SL-HP cells infected for 5 h under starvation (6.5 h). (B) Observation of autolysosomes and autophagosomes using electron microscopy. (B1) Control SL-HP cells cultured in complete medium with 10%FBS, (B2) SL-HP cells starved for 6.5 h, showing autolysosomes; (B3) SL-HP cells infected for 5 h under starvation (6.5 h), showing autolysosomes; (B4) Control Sl-zsu-1cells cultured in complete medium with 10% FBS; (B5 and B6) Sl-zsu-1 cells infected with AfMNPV at 4 h of post infection, showing two or one autophagosomes, respectively. M, mitochondria; AL, autolysosomes; AP, autophagosomes. Bars = 20 µm in Fig. A; Bars = 2 µm in Fig. B.

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