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. 2012 May 21;7(5):e37457. doi: 10.1371/journal.pone.0037457

Figure 5. A shift from autophagy to apoptosis triggered by baculovirus under amino acid deprivation.

Figure 5

The starved SL-HP cells (A1–3) and the infected cells under starvation (B1–3) were shown. (A1) Observation of the cells starved for 48 h using light microscope, (A2) Hoechst staining of the cells starved for 48 h; (A3) DNA agarose electrophoresis showing that no DNA degradation occurred after 0, 24, 48 and 72 h of starvation. (B1) Observation of the cells infected for 24 h using light microscope, (B2) Hoechst staining of the cells infected for 24 h under starvation (25.5 h), (B3) DNA agrose electrophoresis showing that DNA fragmentation occurred in AcMNPV-infected SL-HP cells under starvation at 12 h or 24 h of post-infection, (C1) unstarved SL-HP cells, (C2) unstarved SL-HP cells stained with Hoechst; (C3) DNA extracted from SL-HP cells starved for 24 h was run at 1×, 2× and 4×; (D) Analysis of relative caspase-3 activity, (D1) Starved cells; (D2) AcMNPV-infected cells under starvation condition. *p<0.05. Bars = 20 µm. M, 100 bp DNA ladder.

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