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. 2012 May 21;7(5):e37457. doi: 10.1371/journal.pone.0037457

Figure 8. The expression, localization, abundance and cleavage of Atg6 in insect cells.

Figure 8

(A) the purified BmAtg6-His/V5 expressed in E.coli, (B) LD652 cells transfected with plasmid BmAtg6-GFP and observed with the confocal laser microscopy at 24 h post transfection, showing the localization of over-expressed BmAtg6-GFP protein; The blue light emitted by Hoechst was changed to red light with software, (C) localization of the endogenous SlAtg6 in SL-HP cells shown by immunofluorescence using anti-BmAtg6 mouse serum. (D)Abundance alteration of SlAtg6 under various conditions; lane 1, unstarved Sl-HP cells; lane 2, SL-HP cells starved for 6 h; lane 3, AcMNPV-infected SL-HP cells at 4 h of post-infection; lane 4, AcMNPV-infected SL-HP cells starved for 6 h at 4 h of post-infection, (E) No cleavage of endogenous SlAtg6 was observed in normal SL-HP cells (control) and apoptotic cells induced with actinomycin D (1 µg/ml) by western blot using anti-BmAtg6 serum. Bars = 20 µm.

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