Table 1. PCR primers used in this study.

Primer	Sequencea	Final target
PIZ-Atg6F	gg GGTACC ATG AGT GAT TCA AAG ACG TTC GT	Expression in insect cells
PIZ-Atg6R	gc TCTAGA CCT TGT GGC TCA GAT TTG TCC TC	Expression in insect cells
PET-Atg6F	cgc GGATCC ATG AGT GAT TCA AAG ACG TTCGT	Expression in bacteria
PET-Atg6R	ccc AAGCTT TTG TGG CTC AGA TTT GTC CTC	Expression in bacteria
PIE2-Atg6F	ccc AAGCTT TCA TGA TGA TAA ACA ATG TAT GGT G	Location in insect cells
PIE2-Atg6R	cgc GGATCCCC TTG TGG CTC AGA TTT GTC CTC	Location in insect cells
AcMNPV IE1-F	CGA CGA CAA CGA CTA CAA	Semi-PCR at cDNA level
AcMNPV IE1-R	CTC ACA TAC GGC GAT ACA A	Semi-PCR at cDNA level
AcMNPV P35-F	TCC ACG ATA GCA TCA AGT	Semi-PCR at cDNA level
AcMNPV P35-R	GAG CAA ACG GCA CAA TAA	Semi-PCR at cDNA level
Actin-F	GCG CGG CTA CTC GTT CAC TAC C	Semi-PCR at cDNA level
Actin-R	GGA TGT CCA CGT CGC ACT TCA	Semi-PCR at cDNA level
HaAtg8-F	ccc AA GCT TCG GGC GGT GGA GGG ATG AAA TTC CAA TAT AAA GAA G	Cloning
HaAtg8-R	cg GGATCC TTA ATA TCC ATA TAC ATT CT	Cloning
Total	14	7

^a: The lower-case letters indicate the corresponding protective bases of the restriction enzymes. The underlining letters indicate the cleavage sites of the restriction enzymes.