Table 1.
Predicted antigenicity (antigen value) of the 20 epitopes and the EIA detection results.
| Number | Predicted epitope | Surface probability | Flexibility | β-turn | Coil | Glycosylation site Asn-X-Thr/Ser | Predicted antigen value | EIA A450 value |
|---|---|---|---|---|---|---|---|---|
| (1) | gB258–75 | 5.219 | 44–82 | 69, 71 | 69, 70 | 1.588 | 0.361 ± 0.011 | |
| (2) | gB2466–473 | 5.685 | 465–485 | 473 | 470 | 2.31 | 0.987 ± 0.031 | |
| (3) | gB2468–475 | 4.185 | 465–475 | 473 | 470 | + | 1.593 | 0.276 ± 0.011 |
| (4) | gB2300–306 | 1.984 | 300–306 | 301 | 301–305 | 2.057 | 0.118 ± 0.024 | |
| (5) | gC2326–335 | 1.320 | 328–335 | 330–332 | 333–335 | + | 2.044 | 0.278 ± 0.011 |
| (6) | gC295–105 | 1.315 | 95–100 | 93, 98 | 100–104 | 2.01 | 0.687 ± 0.022 | |
| (7) | gC2161–171 | 1.948 | 161–170 | 167 | 164–170 | + | 1.736 | 0.689 ± 0.021 |
| (8) | gC2216–223 | 2.242 | 216–223 | 217, 222 | 219–223 | 2.235 | 0.898 ± 0.030 | |
| (9) | gE2514–522 | 3.446 | 514–522 | 519 | 515 | 2.322 | 0.655 ± 0.030 | |
| (10) | gE2471–479 | 2.02 | 471–479 | 478 | 471–47 | 2.287 | 0.877 ± 0.031 | |
| (11) | gE2483–491 | 2.972 | 484–495 | 488, 492 | 485, 493 | 2.367 | 1.107 ± 0.042 | |
| (12) | gE2385–398 | 2.701 | 385–398 | 396 | 385–394 | 2.49 | 0.564 ± 0.021 | |
| (13) | gG2526–539 | 2.613 | 526–537 | 535, 539 | 530, 534 | 1.866 | 0.456 ± 0.019 | |
| (14) | gG2286–295 | 1.215 | 289–293 | 290 | 288 | 1.56 | 0.821 ± 0.025 | |
| (15) | gG2572–579 | 2.767 | 569–577 | 569, 574 | 570 | 2.807 | 0.811 ± 0.031 | |
| (16) | gG2350–364 | 1.844 | 350–364 | 351 | 353–358 | 2.124 | 0.992 ± 0.033 | |
| (17) | gI2202–226 | 2.453 | 202–226 | 203, 210, 217 | 205 | + | 1.859 | 0.413 ± 0.011 |
| (18) | gI2236–253 | 2.143 | 236–253 | 242, 253 | 241, 244–9, 251 | 2.081 | 0.856 ± 0.026 | |
| (19) | gI2286–295 | 4.729 | 287–295 | 287, 293 | 2.764 | 1.034 ± 0.039 | ||
| (20) | gI2319–337 | 3.427 | 319–337 | 323, 326, 333 | 321, 325, 335 | 2.042 | 0.569 ± 0.021 | |
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| C | 0.121 ± 0.011 | |||||||