Table 2.

Antigenicity and specificity analysis of the five epitopes using EIA.

Number	Antigen (peptides-BSA)	Antibody to the proteins (titer)	OD value (450 nm)	Antigen (proteins)	Antibody to peptides-BSA (titer)	EIA A450 value
(1)	gB2466–473/BSA	gB2 (1 : 10 000)	0.977 ± 0.039	gB2	gB2466–473/BSA (1 : 5000)	1.099 ± 0.037
(2)	gC2216–223/BSA	gC2 (1 : 10 000)	0.899 ± 0.031	gC2	gC2216–223/BSA (1 : 5000)	1.102 ± 0.040
(3)	gE2483–491/BSA	gE2 (1 : 10 000)	1.102 ± 0.040	gE2	gE2483–491/BSA (1 : 5000)	0.893 ± 0.029
(4)	gG2572–579/BSA	gG2 (1 : 10 000)	0.882 ± 0.011	gG2	gG2572–579/BSA (1 : 5000)	0.986 ± 0.031
(5)	gI2286–295/BSA	gI2 (1 : 10 000)	0.792 ± 0.029	gI2	gI2286–295/BSA (1 : 5000)	0.876 ± 0.031
(6)	BSA	All the proteins# (1 : 10 000)	<0.2000	All the proteins	BSA** (1 : 5000)	<0.2000

^#: BSA did not react with all the antisera to the five proteins.

**: The five proteins did not react with all the antisera to BSA.