Table 3.
Antigenicity and specificity detection of the five peptides using Western Blot analysis.
| Number | Antigen (peptides-BSA) | Antibody to the proteins (titer) | Western blotting | Antigen (proteins) | Antibody to peptides-BSA (titer) | Western blotting |
|---|---|---|---|---|---|---|
| (1) | gB2466–473/BSA | gB2 (1 : 10 000) |
++* | gB2 | gB2466–473/BSA (1 : 5000) | ++ |
| (2) | gC2216–223/BSA | gC2 (1 : 10 000) |
++ | gC2 | gC2216–223/BSA (1 : 5000) | ++ |
| (3) | gE2483–491/BSA | gE2 (1 : 10 000) |
+++ | gE2 | gE2483–491/BSA (1 : 5000) | +++ |
| (4) | gG2572–579/BSA | gG2 (1 : 10 000) |
++ | gG2 | gG2572–579/BSA (1 : 5000) | ++ |
| (5) | gI2286–295/BSA | gI2 (1 : 10 000) |
++ | gI2 | gI2286–295/BSA (1 : 5000) | ++ |
| (6) | BSA | All the proteins# (1 : 10 000) | — | All the proteins | BSA** (1 : 5000) |
— |
*: +++, strong reaction; ++, good reaction; +, moderate reaction; —, no reaction.
#: BSA did not react with all the antisera to the five proteins.
**: The five proteins did not react with the antisera to BSA.