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Sema3A-induced DRG axonal repulsion in vitro. (A–D) Co-culture of DRG explants from control and RhoAf/f; Wnt1-Cre embryos with GFP (A,C), or Sema3A and GFP (B,D) transfected cell aggregates. The axons of DRG neurons were examined by anti-TuJ1 antibody. Both DRG axons from control and RhoAf/f; Wnt1-Cre embryos were repelled by Sema3A. Dotted lines outline aggregates of 293T cells.
