Table 1.
Primary antibody | Clone | Product company | Dilution | Cell type marked |
---|---|---|---|---|
Calbindin (CB) | 300 | Swant | 1:5000 | Localizes D-28k CB which occur in a subset of neurons |
Dab1 | Polyclonal | Sigma-Aldrich (D1569) | 1:1000 | A subset of cell types |
GFAP | GA5 | Chemicon | 1:1000 | Glial fibrillary acidic protein expressed in astrocytes |
Map2a,b | AP20 | Chemicon | 1:1000 | Microtubule associated protein-2 expressed in mature neurons |
NeuN | A60 | Chemicon | 1:1000 | Neuronal nuclei protein |
RC2 | RC2 | Hybridoma-Bank | 1:500 | A 295kDa intermediate filament protein present in radial glial cells |
RIP | NS-1 | Chemicon | 1:1000 | Detects non-myelin and myelin oligodendrocytes |
Reelin | G10 | Chemicon | 1:1000 | Detects reelin expressed in a subset of neurons |
TH | LNC1 | Chemicon | 1:1000 | Detects tyrosine hydroxylase enzyme expressed by dopaminergic neurons |