Table 3.
Gene therapy to protect liver againts ischemia/reperfusion. Heme oxygenase-1, HO-1; interleukin-13, IL-13; small interference RNA, siRNA; superoxide dismutase, SOD; tumour necrosis factor TNF.
| Gene therapy | ||||
|---|---|---|---|---|
| Liver transplantation and warm hepatic ischemia | ||||
| Drug | Specie | Experimental model | Ischemic time | Effect |
| Bag-1 (adenoviral transfer) | Rat | Liver transplantation | 24 h | ↓ TNFα, ↓neutrophil infiltration, ↓ microcirculatory disturbances |
| Bcl-2 gene (adenoviral transfer) | Rat | Liver transplantation | 16 h | ↓ Apoptosis |
| Cu/Zn-SOD gene (adenoviral transfer) | Rat | Liver transplantation | 24 h | ↓ Oxidative stress |
| HO-1 gene (adenoviral transfer) | Rat | Liver transplantation | 4 h | ↓ Macrophage infiltration, ↓ apoptosis |
| IL-13 (adenoviral transfer) | Rat | Liver transplantation | 24 h | ↓ Proinflammatory cytokines, ↓ liver neutrophil recruitment |
| Adiponectin (siRNA) | Rat | Warm ischemia | 1 h | ↓ Oxidative stress |
| ASMase (siRNA) | Mice | Warm ischemia + hepatectomy | 90 min | ↓ Ceramide, ↓ apoptosis |
| Caspase 3 (siRNA) | Mice | Warm ischemia | 90 min | ↓ Apoptosis |
| Caspase 8 (siRNA) | Mice | Warm ischemia | 90 min | ↓ Apoptosis |
| SOD gene (adenoviral transfer) | Rat | Warm ischemia | 1 h | ↓ Oxidative stress |
| IL-13 (adenoviral transfer) | Rat | Warm ischemia | 30 min | ↓ Proinflammatory cytokine, ↓ neutrophil recruitment |