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. 2012 Jan 12;26(1):89–102. doi: 10.3109/01677063.2011.642910

Figure 5.

Figure 5

Increasing reporter gene copy number improves sensitivity of the CaLexA system, (a–e) Measurement of GFP fluorescence intensity in DA1 of female flies after exposed to cVA (20 μg) for 24 hours. The test fly contained GH146-Gal4, UAS-mLexA-VP16-NFAT, and LexAop-CD2-GFP (1 × GFP) or both LexAop-CD2-GFP and LexAop-CD8-GFP-2A-CD8-GFP (3 × GFP). (a–d) Images of GFP fluorescence in antennal lobe, (e) Quantitative analysis of GFP fluorescence in DA1. (f–i) Images of GFP fluorescence in antennal lobe, n = 6–9. (j) Quantitative analysis of GFP fluorescence in DA1. GFP fluorescence intensity is displayed in arbitrary unit, n = 6–9. Error bars indicate standard error of mean. *p < 0.05; **p < 0.01; ***p < 0.001. Wilcoxon signed-rank test. All measurements were obtained from a custom two-photon microscope with the same laser power (61 mW at the back aperture of the objective lens) at the wavelength of 925 nm. Scale bar = 20 μm.