Fig.3.

Rotenone and paraquat treatment for 24 h alters cell viability (MTT assay), GSH content, and reactive oxygen species (ROS) in neurons and astrocytes. The 5 day in vitro cultures of neurons were exposed to rotenone 30nM and paraquat 30μM (fig. 3a.). Confluent astrocytes were exposed to 100nM rotenone and 500μM paraquat (fig. 3b). MTT was assayed as a measure of cell viability based on mitochondrial succinate dehydrogenase activity. GSH was measured using MCB fluorescence. ROS was assayed using DCF fluorescence by FACS. Values are expressed as the mean±SEM. N=3. * denotes P<0.05, treated cells compared to control