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. 2012 Jun 15;139(12):2234–2245. doi: 10.1242/dev.075184

Fig. 3.

Fig. 3.

UNC-4 regulates connectivity in the motor neuron circuit. (A) UNC-4 functions with co-repressor UNC-37 to block expression of the VB gene CEH-12 and to preserve VA-type inputs (blue). (B) De-repression of CEH-12 in posterior VAs in unc-4 and unc-37 mutants results in the mis-wiring of VAs with VB-type inputs (red) and disrupted backward locomotion. EGL-20 promotes CEH-12 expression. (C) Mutations in ceh-12 eliminate ectopic connections with AVB and partially suppress the Unc-4 phenotype. (D-F) Locomotion assays. ‘Unc’ animals cannot crawl backward. ‘Suppressed’ worms show detectable backward movement. ceh-12(gk391) and egl-20(n585) mutants strongly suppress the Unc-4 phenotype of hypomorphic alleles unc-4(e2323) and unc-37(e262) (D) and unc-4(e2322ts) (E). A mig-1(e1787) mutation partially suppresses Unc-4 movement (E). ceh-12 and egl-20 mutants partially suppress the null allele unc-4(e120) (F). *P<0.05, **P<0.01, ***P<0.0001. §P<0.05 versus unc-4; egl-20. n≥50. N.S., not significant.