Fig. 7.

. GRASP markers detect chemical synapses between AVA and A-motor neuron partners. (A,B) AVA synapses with DA and VA motor neurons in wild type. Boxed area shows the posterior region between VA10 and DA7 where AVA synapses specifically with VA10 (B). (C) GRASP-dependent GFP puncta (yellow arrows) with VA10 (red) are reduced in unc-4(e120) and restored by ceh-12(gk391) or egl-20(n585). (D) Intensity values of GFP puncta were quantified from line scans of this region (see Materials and methods). ^*P<0.01, ^**P<0.001, analysis of variance (ANOVA). n≥10 for each neuron.