Table 4.
Description of selected recent diagnostic assessments of dengue RDT combining NS1 antigen, IgM, and IgG antibody results.
| Assay | Study | Year | Location | Sample timing | Reference comparator | Analyte combination | Sensitivity (95% CI) | Specificity (95% CI) |
|---|---|---|---|---|---|---|---|---|
| Tricou et al. [39] | 2010 | Vietnam | 1–6 days illness | RT-PCR or paired MAC and GAC-ELISA | NS1/IgM | 75.5 (69.6 – 80.8) | 100 (93.8 – 100) | |
| NS1/IgM/IgG | 83.7 (78.4 – 88.1) | 97.9 (88.7 – 99.9) | ||||||
| Wang and Sekaran [21] | 2010 | Malaysia | 1–15 days | Virus isolation, RT-PCR, rising titer in a paired samples using MAC ELISA | NS1/IgM | 88.7 (84.0 –93.3) | 98.8 (96.3–100) | |
| SD Dengue Duo Bioline | Osorio et al. [40] | 2010 | Colombia | Median 4; range 2–7 days illness | Viral culture, nested RT-PCR or paired MAC and GAC-ELISA | NS1/IgM | 78.4 (72.4 –83.7) | 91.3 (83.6–96.2) |
| NS1/IgM/IgG | 80.7 (75–85.7) | 89.1 (81–94.7) | ||||||
| Blacksell et al. [19] | 2011 | Sri Lanka | Median 5; IQR 2-7 days illness | AFRIMS MAC and GAC-ELISA paired samples | NS1/IgM | 92.9 (83.9 –97.1) | 88.8 (82.8–93.2 | |
|
| ||||||||
| Panbio Early Rapid NS1 and Duo assay | Fry et al. [20] | 2011 | Malaysia | 1–15 days; 70% ≤5 days illness | RT-PCR or paired MAC and GAC-ELISA | NS1/IgM | 89.0 (85.2 – 92.8) | Not reported |
| NS1/IgM/IgG | 93.0 | Not reported | ||||||
| Blacksell et al. [19] | 2011 | Sri Lanka | Median 5; IQR 2–7 days illness | AFRIMS MAC and GAC-ELISA paired samples | NS1/IgM | 89.9 (82.2–95.0) | 75.0 (67.6–81.5) | |