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. Author manuscript; available in PMC: 2013 May 11.
Published in final edited form as: Cell. 2012 May 3;149(4):923–935. doi: 10.1016/j.cell.2012.03.034

Figure 2. SRGAP2C dimerizes with SRGAP2A and inhibits its membrane deformation properties in COS7 cells.

Figure 2

(A) Co-immunoprecipitation (Co-IP) of SRGAP2A-GFP or SRGAP2C-GFP along with HA-SRGAP2C in HEK293T cells transfected with the indicated constructs. Co-IP was performed using mouse anti-HA antibody and mouse IgG as negative control. Western blotting (WB) was performed with a mouse (ms) anti (α)-GFP antibody.

(B–K) Representative confocal images of COS7 cells transfected with indicated constructs and visualized by EGFP signal. Scale bar: 5 µm. Inset shows 2-fold magnification.

(L) Box plot showing quantification of the number of filopodia per pixel along cell periphery, for cells represented in B–K. n = 20 cells per condition. *** p<0.001, Mann-Whitney test.