Figure 6.

Imaging Lunx and SP-A protein expression in different cancer cells with the ICC method. Following ICC with anti-human Lunx or SP-A Ab, the results were observed in the cytoplasm of different kinds of cancer cells. The brown-colored deposits (arrows) located in the cytoplasm of cancer cells indicates that the cancer cells express the protein. The magnification was 100×. Lunx protein can be expressed in A549 (A) and SPC-A-1 lung cancer cells (B). Lunx protein can not be expressed in HepG2 (C) and HCT-8 cancer cells (D). SP-A protein can be expressed only in A549 lung cancer cells (E). SP-A protein can not be expressed in SPC-A-1 lung cancer cells (F), HepG2 (G) and HCT-8 cells (H).

Abbreviations: ICC, immunocytochemistry; SP-A, surfactant protein-A; Ab, antibody.