Figure 3.

UHRF1 and UHRF2 are enriched at pericentric heterochromatins independent of cell cycle, whereas DNMT1 is enriched at pericentric heterochromatins only in middle and late S phase. NIH3T3 cells were transfected with GFP-UHRF1 or GFP-UHRF2 and blocked at S phase by aphidicolin treatment. The cells were then released from S phase block and labeled with BrdU (red) and also stained with 4′,6-diamidino-2-phenylindole (DAPI). DNMT1 was revealed by immunostaining using a DNMT1 specific antibody. The DAPI staining foci represent pericentric heterochromatins.