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. 2011 Nov 8;21(12):1723–1739. doi: 10.1038/cr.2011.176

Figure 4.

Figure 4

The H3K9me2/3 binding activity is the primary determinant for pericentric heterochromatin association of UHRF2. (A) The pericentric heterochromatin (HC) association for endogenous UHRF2 in NIH3T3 cells. (B) The pericentric heterochromatin localization for wild-type, methylated H3K9-binding deficient (WF143/144AA and FW262/263AA) and hemi-methylated DNA binding deficient (R520A and Y584A) mutants was analyzed in NIH3T3 using anti-Flag antibody cells. After counting large quantity of cells, the percentage of cells with UHRF2 and DAPI co-localization with DAPI foci representing pericentric heterochromatin (HC) was calculated for wild-type UHRF2 and each mutant.