Figure 6.

UHRF2 interacts with DNMT1, DNMT3a, DNMT3b and G9a. (A) UHRF1 co-immunoprecipitated with DNMT1, DNMT3a and DNMT3b. GFP-tagged UHRF1 was expressed alone or together with DNMT1, DNMT3a and DNMT3b, respectively in 293T cells and subjected to immunoprecipitation analysis using anti-Flag antibody and western blot analysis using UHRF1 and Flag antibodies. (B) UHRF2 co-immunoprecipitated with DNMT1, DNMT3a and DNMT3b. The co-immunoprecipitation experiments were performed as above except GFP-UHRF2 was used. (C) UHRF1 and UHRF2 in HeLa nuclear extracts co-immunoprecipitated with DNMT1. Note that low levels of UHRF1 were detected in immunoprecipitation of UHRF2 and vice versa, most likely due to slight cross-reaction in western blot between UHRF1 and UHRF2 antibodies. (D) Both UHRF1 and UHRF2 co-immunoprecipitated with G9a. GFP-tagged UHRF2 or UHRF1 were expressed alone or together with Flag-G9a in 293T cells and subjected to IP-western analysis using antibodies as indicated.