Figure 6.

Confocal micrographs of double-labeled LC3B and NeuN as a ganglion cell marker (top). In the control, punctuate LC3B immunoreactivity was located mainly in the IPL with little LC3B visible in the cytoplasm of RGCs (RGCs; outlined as yellow line) in the GCL. After 2 weeks of IOP elevation, punctuate LC3B immunoreactivity increased in the cytoplasm of RGCs in the GCL. After 4 weeks of IOP elevation, LC3B immunoreactivity markedly increased in the cytoplasm of RGCs in the GCL and immunoreactivity was decreased in the IPL, where dendrites of RGCs are located. Three eyes used in each experimental period. Scale bars: 50 μm