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Confocal micrographs of double-labeled LC3B and TUNEL staining. Most of the TUNEL-positive RGCs showed co-staining with LC3B (arrow). However, there was TUNEL-positive RGCs without increased staining of LC3B in the cytoplasm of the RGCs (arrowhead) at all period. Three eyes used in each experimental period. Scale bars: 50 μm
