Figure 2.
Influence of RITA and nutlin-3 on p53 activation and apoptosis in UKF-NB-3, UKF-NB-3rNutlin10μM, and UKF-NB-3rRITA10μM cells. (a–b) Cells were treated with RITA (10 μM) or Nutlin-3 (10 μM) for 24 h and investigated by western blot for the expression of p53 target genes (a) or for the formation of the cleaved form of PARP (cPARP) (b). β-actin served as loading control. (c) Cells were treated with RITA (10 μM) or Nutlin-3 (10 μM) for 24 h. Caspase 3 activation was determined by Caspase-Glo 3/7 Assay. *P<0.05