Figure 3.

Mean phage production (closed squares) and mean bacterial densities (growth) of UM phenotypes over time with (dashed line, open circles) and without phage (black straight line, closed circles) (a). After two hours only FM phenotypes were detected after co-inoculating initially UM phenotypes with sympatric phage populations (all REVs were readily lysed); however phage production continued, showing that although mucoid phenotypes are sensitive to phage they can continue to grow and increase in cell density in their presence. The effect of phage on non-mucoid bacteria cell densities (growth) through time (b). The ancestral SM phenotype of SBW25 was grown both in the absence (straight black line, closed circles) and in the presence (dashed lines, open circles) of three different populations of phage from Treatment 1. Cell densities are greatly reduced in non-mucoid phenotypes in the presence of phage compared with mucoid phenotypes.