Table 2. Annotation of genes of fosmids MMCf1 and MMCf2.
| MMCf1 ORF no. (aa) | MMCf2 ORF no. (aa) | Predicted protein | MMCf1 closest homologue Uniprot BLASTp E-value <1e-20 | MMCf2 Closest homolog Uniprot BLASTp E-value <1e-20 |
|---|---|---|---|---|
| 1 (212) | Peroxidase (EC 1.11.1.7) | Haliangium ochraceum DSM 14365 3e-88 | ||
| 2 (143) | Putative thioesterase superfamily protein | Myxococcus xanthus DK 1622 2e-23 | ||
| 3 (91) | Hypothetical protein | — | ||
| 4 (131) | Hypothetical protein | — | ||
| 5 (134) | Hypothetical protein, polyketide cyclase/dehydrase-like | — | ||
| 6 (185) | Transcriptional regulatory protein | P. pacifica SIR-1 1e-28 | ||
| 7 (88) | Conserved hypothetical protein DUF1876 family | — | ||
| 8 (243) | Conserved hypothetical protein, metallo-hydrolase/oxidoreductase superfamily protein-like | Geodermatophilus obscurus DSM 43160 1e-85 | ||
| 9 (318) | Putative aldo/keto reductase, containing potassium channel β-chain regulatory domain | Uncultured bacterium Bio6 e-110 | ||
| 10 (705) | Putative extracellular matrix protein, Dictyostelium discoideum (slime mold) prestalk protein-like | Dictyostelium minutum 4e-26 | ||
| 1 | 11 | 16S rRNA gene | ||
| 2 (470) | 12 (470) | mauG: methylamine utilisation protein mauG | Alcanivorax sp. DG881 2e-119 | Alcanivorax sp. DG881 1e-113 |
| 3 (148) | 13 (152) | Putative MarR transcriptional regulator | — | — |
| 4 (293) | 14 (303) | C-5 sterol desaturase (EC 1.3.-.-) | Flavobacteriales bacterium ALC-1 8e-87 | Flavobacteriales bacterium ALC-1 2e-87 |
| 5 (1475) | 15 (1670) | Conserved hypothetical protein associated with cell–cell interaction, containing Myxococcus cysteine-rich repeat | H. ochraceum DSM 14365 3e-22 | H. ochraceum DSM14365 2e-31 |
| 6 (490) | 16 (490) | gltB: glutamate synthase (NADPH) small chain (EC 1.4.1.13) | Blastopirellula marina DSM 3645 0.0 | Blastopirellula marina DSM 3645 0.0 |
| 7 (1532) | 17 (1532) | gltA: glutamate synthase (NADPH) large chain (EC 1.4.1.13) | Blastopirellula marina DSM 3645 0.0 | Blastopirellula marina DSM 3645 0.0 |
| 8 (176) | 18 (176) | AsnC/Lrp-family transcriptional regulatory protein | M. xanthus DK 1622 2e-28 | Sorangium cellulosum 4e-28 |
| 9 (412) | 19 (412) | Protein kinase (EC 2.7.-.-) | S. cellulosum 1e-76 | S. cellulosum 4e-77 |
| 10 (255) | 20 (255) | Serine protease, trypsin family, histidine active site | — | — |
| 11 (429) | 21 (402) | Predicted DD-peptidase family peptidoglycan-binding protein | H. ochraceum DSM 14365 9e-45 | P. pacifica SIR-1 2e-29 |
| 12 (207) | 22 (207) | Ham1-family nucleoside triphosphatase (EC 3.6.1.15) | Pseudovibrio sp. JE062 8e-34 | Pseudovibrio sp. JE062 4e-41 |
| 13 (800) | 23 (800) | Conserved hypothetical protein, YyaL-like | Desulfobacterium autotrophicum 0.0 | D. autotrophicum 0.0 |
| 14 (320) | 24 (320) | Conserved hypothetical protein | H. ochraceum DSM 1436 2e-41 | H. ochraceum DSM 14365 7e-42 |
| 15 (327) | 25 (327) | Mandelate racemase/muconate lactonising enzyme | Sulfitobacter sp. NAS-14.1 8e-78 | Oceanicola batsensis strain HTCC2597 3e-78 |
| 16 (262) | 26 (262) | Hypothetical protein hydrolase-like | Planctomyces maris DSM 8797 1e-33 | Planctomyces maris DSM 8797 1e-31 |
| 17 (401) | 27 (401) | Hypothetical protein | — | — |
| 18 (161) | 28 (161) | Flavin reductase-like, FMN binding | Chloroflexus aurantiacus DSM 637 1e-28 | Chloroflexus aurantiacus DSM 637 2e-28 |
| 19 (228) | 29 (228) | Phosphoglycerate mutase family protein | Thermus thermophilus HB8 1e-27 | T. thermophilus HB8 2e-28 |
| 20 (341) | 30 (341) | Predicted cytochrome c551 peroxidase (EC 1.11.1.5) | Parabacteroides johnsonii 2e-86 | Parabacteroides johnsonii 8e-87 |
| 21 (633) | 31 (633) | Putative activator of 2-hydroxyglutaryl-CoA dehydratase | S. cellulosum 3e-164 | S. cellulosum 3e-162 |
| 22 (1183) | Putative CoA-substrate-specific enzyme activase | S. cellulosum 0.0 | ||
| 23 (395) | Saccharopine dehydrogenase-like oxidoreductase | H. ochraceum DSM 14365 3e-178 | ||
| 24 (202 truncated) | Predicted carboxynorspermidine decarboxylase (truncated) | S. fumaroxidans DSM 10017 7e-54 |
Abbreviations: FMN, flavin mononucleotide; MMC, marine myxobacteria cluster; NADPH, nicotinamide adenine dinucleotide phosphate; ORF, open reading frame.