Fig. 2.

HCV Core-treated CD33⁺ cells suppress CD4 and CD8 T-cell proliferation and IFN-γ production. Human PBMCs were treated with HCV core or β-gal for 7 days. CD33⁺ cells were then cocultured with CD4 or CD8 T cells as described above. (A,B) CFSE dilution was then assessed as a measure for proliferation. (C,D) Cells were restimulated with PMA/ionomycin and intracellular expression of IFN-γ was determined. (E,F) Supernatants were collected following 3 days of CD33-T cell coculture and IFN-γ production was measured by ELISA. *P < 0.05.