Fig. 3.

HCV core-treated CD33⁺ cell mediated suppression is contact-dependent. Human PBMCs were treated with HCV core or β-gal for 7 days. CD33⁺ cells were then cocultured with CD4 or CD8 T cells as previously described for 3 days in transwell plates. (A,B) CFSE dilution was assessed as a measure for proliferation. (C,D) Cells were restimulated with PMA/ionomycin and intracellular expression of IFN-γ was determined. (E,F) Supernatants were collected following 3 days of CD33-T cell coculture and IFN-γ production was measured by ELISA.