Table A1. Coxiella burnetii MST genotypes from humans and ruminants sampled during the Q fever outbreak, the Netherlands, 2008–2010*.
| Sample no. | Host | Source | Location | Year | Ct value | MST genotype† | Cox2 | Cox5 | Cox18 | Cox20 | Cox22 | Cox37 | Cox51 | Cox56 | Cox57‡ | Cox61 |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Q001 | Sheep | Vaginal swab | 1 | 2008 | 25.7 | 33 | 7 | 5 | 1 | –§ | 5 | 9 | 9 | 4 | 3 | 2 |
| Q002 | Sheep | Vaginal swab | 1 | 2008 | 16.3 | 33 | 7 | 5 | 1 | 6 | 5 | 9 | 9 | 4 | 3 | 2 |
| Q003 | Sheep | Vaginal swab | 1 | 2008 | 18.8 | 33 | 7 | 5 | 1 | 6 | 5 | 9 | 9 | 4 | 3 | 2 |
| Q004 | Lamb | Throat swab | 1 | 2008 | 27.9 | 33 | 7 | 5 | 1 | 6 | 5 | 9 | 9 | 4 | 3 | 2 |
| Q005 | Lamb | Throat swab | 1 | 2008 | 29.9 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q006 | Lamb | Throat swab | 1 | 2008 | 28.9 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q050 | Human | BAL | 2 | 2009 | 22.4 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q052 | Human | Sputum | 3 | 2009 | 20.7 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q054 | Human | Sputum | 3 | 2009 | 19.4 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q057 | Human | Sputum | 3 | 2009 | 20.6 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q063 | Human | Sputum | 4 | 2009 | 29.6 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | – | 2 |
| Q066 | Human | Sputum | 5 | 2009 | 27.7 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | – | 2 |
| Q076 | Human | Aorta valve | 6 | 2009 | 17.0 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q084 | Human | Aorta valve | 7 | 2008 | 17.0 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q107 | Human | Aorta valve | 5 | 2010 | 9.0 | 33 | 7 | 5 | 1 | 6 | 5 | 9 | 9 | 4 | 3 | 2 |
| Q085 | Goat | Placenta | 8 | 2009 | 18.0 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q087 | Goat | Placenta | 9 | 2009 | 18.1 | 33 | 7 | 5 | 1 | – | 5 | 9 | 9 | 4 | 3 | 2 |
| Q086 | Goat | Placenta | 9 | 2009 | 18.0 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | 6 | 5 |
| Q097 | Cattle | Swab | 10 | 2009 | 19.0 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | 6 | 5 |
| Q090 | Cattle | Milk | 11 | 2010 | 32.0 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | – | 5 |
| Q091 | Cattle | Milk | 12 | 2010 | 32.6 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | – | 5 |
| Q093 | Cattle | Milk | 13 | 2010 | 31.7 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | – | 5 |
| Q096 | Cattle | Milk | 14 | 2010 | 33.4 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | – | 5 |
| Q123 | Cattle | Milk | 15 | 2010 | 31.6 | 20 | 3 | 2 | 6 | – | 5 | 4 | 4 | 10 | – | 5 |
| Q056 | Human | BAL | 16 | 2010 | 28.2 | New | 3 | 3 | 2 | – | – | 9 | – | – | – | – |
| Dugway | NA | CP000733# | NA | NA | NA | 20 | 3 | 2 | 6 | 1 | 5 | 4 | 4 | 10 | 6 | 5 |
| RSA331 | NA | CP000890# | NA | NA | NA | 18 | 3 | 8 | 1 | 6 | 3 | 4 | 7 | 9 | 6 | 3 |
| RSA493 | NA | AE016828# | NA | NA | NA | 16 | 3 | 8 | 5 | 3 | 4 | 1 | 6 | 7 | 6 | 5 |
| CbuG Q212 | NA | CP001019# | NA | NA | NA | 21 | 2 | 1 | 4 | 6 | 2 | 3 | 1 | 11 | 1 | 1 |
| CbuK Q154 | NA | CP001020# | NA | NA | NA | 8 | 5 | 4 | 2 | 5 | 1 | 5 | 3 | 3 | 4 | 4 |
*MST, multispacer sequence typing; Ct, cycle threshold; BAL, bronchoalveolar lavage; NA, not applicable. †MST genotypes were identified by using the MST database (http://ifr48.timone.univ-mrs.fr/MST_Coxiella/mst). ‡Result obtained by using improved amplification primers for Cox57 (9). § –, no result was obtained. The lack of results may be explained by the significantly larger PCR product that is targeted, low quantity of DNA or to overall poor performance of the PCR amplification. ¶This combination of 4 alleles has not been observed and justifies the assignment of a new MST genotype. #GenBank accession number.