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. Author manuscript; available in PMC: 2012 Sep 9.
Published in final edited form as: Dis Aquat Organ. 2011 Sep 9;96(2):145–156. doi: 10.3354/dao02375

Fig. 3.

Fig. 3

Pseudoloma neurophilia spore extraction method comparison by quantification cycle (Cq). NT: no treatment, M: MoBio, Q: QIAgen, P: peroxide, P+M: peroxide + Mobio, P+Q: peroxide + QIAgen, S: sonication, S+M: sonication + Mobio, S+Q: sonication + QIAgen, B+Q: bead beating + QIAgen, C+Q: chitinase + QIAgen. Bars represent mean Cq; points represent actual Cq for individual samples. Sonication treatment alone showed the lowest mean Cq in Trial A, 26.74 followed by sonication with DNA extraction by QIAgen silica gel membrane method (mean Cq = 27.73). Trial B results showed the lowest mean Cq for sonication alone, 28.74, followed by sonication and QIAgen DNA extraction combined, 29.93.