Table 1.
Experimental setup for two extraction comparison trials. Trial A samples represent 300 μl aliquots taken from an initial pool of 30,000 spores in 900 μl of PBS. Following pretreatment, samples were divided into 6 aliquots for DNA extraction. Each extraction method was performed twice. Samples in Trial B represent individual purified spore suspensions containing 1,000 spores each in 100 μl PBS. Methods in Trial B were performed in triplicate.
| Trial | Sample | Pretreatment | DNA Extraction |
|---|---|---|---|
| A | 1 | NT | No Extraction |
| QIAgen | |||
| MoBio | |||
| 2 | Hydrogen Peroxide | No Extraction | |
| QIAgen | |||
| MoBio | |||
| 3 | Sonication | No Extraction | |
| QIAgen | |||
| MoBio | |||
| B | 1 | None | None |
| 2 | Bead Beating | QIAgen | |
| 3 | Chitinase | QIAgen | |
| 4 | None | QIAgen | |
| 5 | None | MoBio | |
| 6 | Sonication | None | |
| 7 | Sonication | QIAgen | |