Table 3.
Comparison of Pseudoloma neurophilia detection by new real-time PCR assay utilizing sonication pretreatment of samples with conventional PCR assay described by Whipps and Kent (2006). The real-time PCR assay described detected the parasite from one to three log dilutions higher than the conventional assay (+ = P. neurophilia ssrDNA detected, - = No P. neurophilia ssrDNA detected).
| Individual | Conventional PCR | Real-time PCR | ||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| 0 | -1 | -2 | -3 | -4 | 0 | -1 | -2 | -3 | -4 | |
| 1 | + | - | - | - | - | + | + | + | - | - |
| 2 | + | - | - | - | - | + | - | - | - | - |
| 3 | + | + | - | - | - | + | + | + | + | - |
| 4 | + | - | - | - | - | + | + | + | - | - |
| 5 | + | - | - | - | - | + | + | - | - | - |
| 6 | + | - | - | - | - | + | + | - | - | - |