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. 2012 Apr 26;12:14. doi: 10.1186/1472-6750-12-14

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Copyright ©2012 Mortimer et al.; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Western blot analysis of H5 and H5tr transient expression in N. benthamiana, showing accumulation in various subcellular compartments 7 days post infiltration. HA protein was detected using a primary rabbit anti-H5N1 polyclonal antibody. Crude plant extracts were analysed from plant tissue infiltrated with Agrobacterium strains carrying the following expression vectors: Lane 1, pTRAERH-H5; lane 2, pTRAERH-H5tr; lane 3, pTRAc-H5; lane 4, pTRAc-H5tr; lane 5, pTRACTP-H5; lane 6, pTRACTP-H5tr; lane 7, pTRAa-H5; lane 8, pTRAa-H5tr; lane 9, protein ladder (Fermentas). The arrow indicates the position of the H5 and H5tr proteins.