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. 2012 May 22;7(5):e37600. doi: 10.1371/journal.pone.0037600

Figure 1. FGF signaling is necessary for endothelial cell monolayer integrity.

Figure 1

A. Endothelial monolayer permeability evaluated with the ECIS system. Transendothelial resistance was measured every 5 minutes for 20 hours after the onset of adenoviral transduction. The experiment reported has been performed 3 times with comparable results. Note that cells lacking FGF signaling showed decreased transendothelial resistance (increased endothelial permeability). B, C, D. VE-cadherin was absent from cell-cell contacts in cells lacking FGF signaling. Immunostaining of quiescent and fully confluent BAEC transduced with Ad-GFP (B) or Ad-FGFR1DN (C: MOI = 10, D: MOI = 100). Cells were stained for VE-cadherin (red) and p120-catenin (green). Note that VE-cadherin was absent from cell-cell contacts (arrows in C) and many gaps were formed (arrows in D) in the monolayer transduced with Ad-FGFR1DN. Scale Bars: 10 µm.