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. 2012 May 22;7(5):e37601. doi: 10.1371/journal.pone.0037601

Figure 6. miR-34a inhibits tumor angiogenesis by downregulating VEGF secretion from tumor cells.

Figure 6

A-B: Paraffin embedded tumor samples were stained with anti-CD31 antibody and microvessel density was calculated by counting 5 random high power fields (200x). A: Representative photographs of UM-SCC-74A tumors expressing scrambled control microRNAs (SC) or miR-34a. B: Microvessel density in tumor samples from scrambled control microRNAs (SC) or miR-34a groups. *, represent a significant inhibition (p<0.05) of tumor angiogenesis in miR-34a group as compared to SC. C: VEGF levels (pg/ml per 106 tumor cells) in culture supernatants from UM-SCC-74A cells transfected with miR-34a or scrambled control microRNA (SC). D: Representative photographs of UM-SCC-74B tumors expressing scrambled control microRNAs (SC) or miR-34a. E: Microvessel density in tumor samples from scrambled control microRNAs (SC) or miR-34a groups. F: VEGF levels (pg/ml per 106 tumor cells) in culture supernatants from UM-SCC-74B cells transfected with miR-34a or scrambled control microRNA (SC).