Table 1. Primer and probe sequences and characteristics.

Gene and Genbank (Accession ID)	Primers and Probes	Sequences	Melting Temperature (°C)	Location on Gene Sequence (base pairs)
SLC2A1	Forward	5′-TCC CAG TGG CCC AAG GA-3′	60	1202–1218
(U89029)	Reverse	5′-GAT CTA TCA GTT TGA GAG TCT CAT CCA-3′	58	1239–1265
	Probe	6FAM-TCA GAG CGC AGG CAG-MGBNFQ		1223–1237
SLC2A3	Forward	5′-TGC CTT ATG GGA TTC TGC AAA-3′	59	431–451
(NM_001009770)	Reverse	5′-TCA GTC GGC CCA AAA TCA G-3′	58	473–491
	Probe	6FAM-AGC AGA GTC AGT TGA AAT-MGBNFQ		454–471
SLC2A4	Forward	5′-AAC CCA GCA CAG AAC TGG AGT AC-3′	59	71–93
(AB005283)	Reverse	5′-CCT GTG TGG ACC CTC AGT CA-3′	58	110–130
	Probe	5′-AGGGCCGGATGAGA-3′		96–109
SLC38A4	Forward	5′-TTG TTT ACA CTG AAG GAG CTT GTG T-3′	59	211–235
(XM_605868)	Reverse	5′-CAG TTC CAT GGG ATC CAT TTG-3′	58	260–280
	Probe	6FAM-CAG CGC TTT CTT GTC C-MGBNFQ		238–253
SLC7A1	Forward	5′-CCT AGC GCT CCT GGT CAT CA-3′	60	271–290
(AF212146)	Reverse	5′-GGC GTC CTT GCC AAG TAC A-3′	58	307–325
	Probe	6FAM-CCT TCT GCA TGG CAG C-MGBNFQ		291–306
SLC7A5	Forward	5′-CTC CAT CCT CTC CAT GAT CCA-3′	58	125–145
(AY162432)	Reverse	5′-TGT GAA CAC CAG TGA GGG TAC AG-3′	59	163–185
	Probe	6FAM-CCG CGG CTG CTG A-MGBNFQ		147–159
SLC7A8	Forward	5′-GTC AGA GCC CGT GGT GTG T-3′	58	205–223
(AY162433)	Reverse	5′-TGC CAG TAA ATA CCC AGG AAG TAG A-3′	59	261–285
	Probe	6FAM-CCA TCA TGG TGA CAG GG-MGBNFQ		237–253
mTOR	Forward	5′-CTG CAC GTC AGC ACC ATC A-3′	59	3772–3790
(XM_001788228)	Reverse	5′-AGC CAT TCC AAC CAA TCA TCT T-3′	58	3830–3851
	Probe	6FAM-CCT CCA AAA GGC C-MGBNFQ		3792–3804
Cyclophilin A	Forward	5′- GTA CTG GTG GCA AGT CCA TCT -3′		105–125
(AY251270.1)	Reverse	5′- CAG GAC CTG TAT GCT TCA GAA TGA -3′		153–176
	Probe	6FAM- ATG GCG AGA AAT TTG-MGBNFQ		126–140
Beta actin	Forward	5′- ACC AGT TCG CCA TGG ATG ATG -3′		76–96
(NM_001009784.1)	Reverse	5′- CCG GAG CCG TTG TCA AC -3′		113–128
	Probe	6FAM- ACG AGC GCA GCA ATA T –MGBNFQ		97–112
GAPDH	Forward	5′- GGG CTG CTT TTA ATA CTG GCA AA -3′		91–114
(NM_001190390)	Reverse	5′- CAT GTA GAC CAT GTA GTG AAG GTC AA -3′		146–171
	Probe	6FAM- CAT CGT TGC CAT CAA TG –MGBNFQ		120–136
YWHAZ	Forward	5′- GAG GGT CGT CTC CAG TAT TGA G -3′		25–46
(AY970970.1)	Reverse	5′- TTC TCG AGC CAT CTG CTG TTT T -3′		70–91
	Probe	6FAM- CAG CAC CTT CCG TCT TT –MGBNFQ		49–65
HPRT1	Forward	5′- AGG TGT TTA TTC CTC ATG GAC TAA TTA TGG -3′		1–30
(EF078978)	Reverse	5′- CAC CCA TCT CCT TCA TCA CAT CTC -3′		52–75
	Probe	6FAM- ACA GGA CCG AAC GAC TG –MGBNFQ		31–47
RPL19	Forward	5′-CAA AAA CAA GCG GAT TCT CAT G -3′		340–361
(AY158223.1)	Reverse	5′- GCT TCT TGC GAG CCT TGT CT -3′		385–404
	Probe	6FAM- AAC ATA TCC ACA AGC TGA A –MGBNFQ		363–381
18S	Eukaryotic 18S rRNA endogenous VIC/MGB Probe and Primers (Applied Biosystems, Foster City, CA, USA)